The cultivation of aloe vera can be carried out by means of tissue culture. Generally, in the sunny days, it is preferable to dig out the sprouts from the field and grow a robust and disease-free aloe vera, remove the dirt and old leaves, and wrap it in paper to bring it back indoors. The long rhizomes and leaves were trimmed and placed in a 1% detergent solution with Tween-80 soaked for 30 minutes. After washing with clean water, they were transferred to a clean bench and placed in 0111% HgCl2 for 8 minutes. Sterile water was used. Rinse 4 times, place in a sterile Petri dish and trim the roots and leaves again. When cultivating, intelligent lighting incubators are used to simulate climatic conditions and provide an effective environment for the smooth progress of research. Add WeChat, you want to have:
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On the clean bench, the treated explants are connected to the culture medium (1), (2), (3), (4), (5), and each bottle is followed by an explant. Incubation in the incubator. On the 7th day of culture, the sprouts of the explants in the medium (3) were sprouted; on the 11th, (2), (4), (5) explants, the buds sprouted; while in the (1) the middle buds were in the first position. Only 15 days after germination, the axillary buds in medium (3) have been elongated by 3-5 cm, and there are 5-7 sprouts of axillary buds on an explant. This indicates that the medium (3) is the starting point of aloe vera explants. Good culture medium. Take the explants on the clean bench, cut out the axillary buds with a length greater than 5cm, and then attach them to the fresh medium (2), (3), (4), and cultivate in a light incubator. After 15 days of culture, there were clustered shoots, in which medium (2) clustered buds averaged 213; (3) 517; (4) 912. Illustrative medium (4) was the best medium for proliferation.
Through the application of the intelligent light incubator, because the chromosomes within the nucleus of Aloe can not perform normal meiosis, it can not produce seeds that can germinate. The conventional propagation is based on sucking buds for asexual reproduction, and the general reproduction speed can only increase by 3 to 5 per year. Times, far from meeting production needs; the conventional propagation cycle of tissue culture is as long as 30-35 days, and it can only breed for 8 to 10 generations a year; the propagation coefficient is only 3 to 5 times. This research result greatly shortens the breeding cycle and improves The propagation coefficient, therefore, can produce aloe seedlings in large quantities and at high speed, and has a broad application prospect in production. 
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